Sinan Soylu; Zeynep Deniz Sahin Inan
Volume 20, Issue 9 , 2018, Pages 1-7
Abstract
Background: Sepsis and multiple organ dysfunction syndrome (MODS) are life-threatening conditions common in intensive care units. In this regard, studies have shown that Strontium ranelate has anti-inflammatory activity by blocking tumor necrosis factor- alpha (TNFα). Objectives: This study aimed ...
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Background: Sepsis and multiple organ dysfunction syndrome (MODS) are life-threatening conditions common in intensive care units. In this regard, studies have shown that Strontium ranelate has anti-inflammatory activity by blocking tumor necrosis factor- alpha (TNFα). Objectives: This study aimed to investigate the effect of Strontium ranelate on MODS in an experimental sepsis model. Methods: The study protocol was approved by Cumhuriyet University Institutional Ethics Committee for Animal Experiments (Sivas-Turkiye, date 07/12/2017). Twenty female Wistar-Albino rats were randomly divided into four groups of sham operation, ce- cal ligation and perforation (CLP), CLP + Strontium (S) (oral 40 mg/kg for 7 days), and S (40 mg/kg oral preoperative 5 days) + CLP + S (oral 40 mg/kg for 7 days). Blood samples were taken, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), bloodurea nitrogen (BUN), and creatinine were studied. Tissues were removed, and inflammation scores were determined.Results: The mean inflammation scores of lung, liver, and renal tissues were found to be the lowest in the sham group (0.8 ± 0.45), and they increased in the S + CLP + S (1.6 ± 0.55) and CLP + S (3.4 ± 0.55) groups, respectively, with the highest score in the CLP (3.8 ± 0.45) group. It was found that there was no statistical difference between the sham and S + CLP + S groups (P > 0.05); however, there was a significant difference between the other groups (P < 0.05). The mean ALT, AST, BUN and creatinine values were found to be the lowest in the sham group, and they increased in the S + CLP + S and CLP + S groups, respectively, with the highest score in the CLP group. Regarding the mean ALT results, it was noted that there was no significant difference between the sham and S + CLP + S groups (P > 0.05); however, there was a significant difference between the other groups in terms of mean ALT, and there was asignificant difference between all the groups in terms of mean AST, BUN, and creatinine (P < 0.05).Conclusions: It was concluded that Strontium ranelate reduced the development of life-threatening MODS in patients with sepsis, especially when it was administered before the development of sepsis, by suppressing inflammatory mediators.
Sinan Soylu; Caglar Yildiz; Birkan Bozkurt; Savas Karakus; Begum Kurt; Atilla Kurt
Volume 20, Issue 3 , 2018, Pages 1-8
Abstract
Background: Incisional hernia repairs are among common abdominal wall surgeries, can be primarily required or being recon- structed using a synthetic or biological material. Objectives: This study aimed at evaluating intra-abdominal adhesions and incisional site healing after the repair of the abdominal ...
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Background: Incisional hernia repairs are among common abdominal wall surgeries, can be primarily required or being recon- structed using a synthetic or biological material. Objectives: This study aimed at evaluating intra-abdominal adhesions and incisional site healing after the repair of the abdominal wall by fresh amniotic membrane-coated polypropylene mesh in comparison to only polypropylene mesh in an experimental rat study. Methods: The study protocol was approved by the Cumhuriyet University Institutional Ethics Committee for Animal Experiments(Sivas-Turkiye, date 24/06/2015). Sixteen pregnant female Wistar-Albino rats (mean weight, 275 g) were anesthetized on the 21st day of pregnancy, and a 1-cm area of the abdominal wall was excised. The pregnancy was terminated, emerging amniotic membranes were dissected, and eight pieces of the 1-cm2 polypropylene mesh were coated with these amniotic membranes without using any suture or adhesive. The polypropylene meshes were sutured on the abdominal wall of eight rats (control group), selected by simple random sampling. For the remaining eight rats, the same procedure was applied with the amniotic membrane-coated polypropy- lene meshes (experimental group). On the 28th postoperative day, the anterior abdominal wall was opened, and intra-abdominal adhesions were assessed macroscopically by Nair’s adhesion scoring system. Strip-shaped biopsy samples were taken from incision lines for histopathological examination.Results: The experimental group had significantly less intra-abdominal adhesions (i.e., Nair’s score of 2 to 4) compared to the con- trol group (two and six rats, respectively; P = 0.046), and had significantly lower mean score for polymorphonuclear leukocyte infil- tration (P = 0.039), hyperemia (P = 0.039), and epithelialization (P = 0.039). The score for the increase in connective tissue (P = 0.018) was significantly higher in the experimental group, and the scores for edema (P = 0.590) and macrophage infiltration (P = 0.590) were similar between the two groups. Conclusions: The use of polypropylene mesh coated with fresh amniotic membrane provides the advantage of decreasing postop- erative intra-abdominal adhesions along with less inflammation and higher epithelialization after abdominal wall repair surgery.
